Detect IGF1R function in postnatal bone marrow mesenchymal stem cell

1. IGF1R signaling pathway is highly enriched in P0.5 BMMSC compared to adult BMMSC; 2. Niche factors are highly expressed in PDGFRa+Sca1+ (PaS) cells. 3. Knockout IGF1R in Prx1+ BMMSC significantly impaired niche factors expression in P0.5 BMMSC. Flow sorted adult PDGFRa+ adult BMSCs, perinatal PDGFRa+Sca1+ BMSCs, perinatal PDGFRa+Sca1- cells, and PDGFRa+Sca1+ perinatal BMSCs with or without IGF1R deletion for RNAseq.

1. 与成年骨髓间充质干细胞（BMMSC）相比，出生后0.5天（P0.5）的骨髓间充质干细胞中，胰岛素样生长因子1受体（IGF1R）信号通路的富集水平显著更高；2. 血小板衍生生长因子受体α（PDGFRα）阳性、干细胞抗原1（Sca1）阳性（简称PaS）的细胞中，龛因子的表达量显著上调；3. 在成对相关同源框蛋白1（Prx1）阳性骨髓间充质干细胞中敲除IGF1R，可显著降低出生后0.5天骨髓间充质干细胞中龛因子的表达水平；本研究通过流式分选获取成年PDGFRα阳性骨髓间充质干细胞、围产期PDGFRα阳性Sca1阳性骨髓间充质干细胞、围产期PDGFRα阳性Sca1阴性细胞，以及分别敲除或未敲除IGF1R的围产期PDGFRα阳性Sca1阳性骨髓间充质干细胞，用于RNA测序（RNA-seq）。