Data from: Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis

This archive contains raw data and code associated to the following publication: Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis Plessier and Marlow, 2025, Preprint at bioRvix This archive is available for download here on zenodo Folders content: - _raw/: raw scRNA-Seq data matrix file used as the primary data source - _SeuratObjects/: processed and annotated Seurat objects (global KikGR+ cells clustering, each line-specific reclustering, progenitor-specific reclustering, and cnidocyte-specific reclustering) - _code/: contains the scripts for processing and analysis of scRNA-Seq data - _input/: contains input files required to run the scripts - _output/: output plots generated by the code Sequencing data availability: - Raw sequencing reads for the scRNA-Seq dataset are available in the GEO repository GSE288441. These include paired-end fastq files for each of the 84 scRNA-Seq library in the paper, alongside the reference genome from the Darwin Tree of Life Consortium from the Wellcome Sanger Institute Tree of Life programme, NCBI GCF_932526225.1 RefSeq assembly and associated NCBI RefSeq gene models jaNemVect1.1 GTF with the KikGR transgene sequence added to both. These also include cell metadata information and raw and filtered global count tables.本归档文件包含与下述学术成果相关的原始数据与代码： 《海葵神经命运特化的细胞与转录轨迹揭示两种成体神经发生模式》 Plessier与Marlow，2025年，预印本发布于bioRxiv 本归档文件可于zenodo平台下载。 文件夹内容如下： - _raw/：作为核心数据源的原始单细胞RNA测序（single-cell RNA Sequencing, scRNA-Seq）数据矩阵文件 - _SeuratObjects/：经过处理与注释的Seurat对象（涵盖全局KikGR+细胞聚类、各谱系特异性重聚类、祖细胞特异性重聚类以及刺细胞特异性重聚类结果） - _code/：包含单细胞RNA测序数据处理与分析的脚本文件 - _input/：包含运行分析脚本所需的输入文件 - _output/：包含代码生成的可视化输出图件 测序数据可获取性说明： 本数据集的原始测序读数可于GEO数据库（登录号GSE288441）获取，包含本文中84个单细胞RNA测序文库的双端fastq文件；同时包含威康桑格研究所生命之树项目达尔文生命之树联盟（Darwin Tree of Life Consortium）提供的参考基因组：NCBI GCF_932526225.1 RefSeq组装版本，以及配套的NCBI RefSeq基因模型jaNemVect1.1 GTF文件，两者均已添加KikGR转基因序列。此外还包含细胞元数据信息，以及原始与过滤后的全局计数表格。