mRNA-seq of Physcomitrella protonema responding to 1 hr red light in wild type and the phytochrome-deficient mutant

The red/far-red light photoreceptor phytochrome mediates photomorphological responses in plants. For light sensing and signaling, phytochromes need to associate with open-chain tetrapyrrole molecules as the chromophore. Biosynthesis of tetrapyrrole chromophores requires members of ferredoxin-dependent bilin reductases (FDBRs). There are two FDBRs in Physcomitrella patens, HY2 and PUBS. Knocking out both generates the phytochrome-deficient mutant. Datasets here provides the transcriptome profiling of Physcomitrella protonema grown in the dark and exposed to one hour red light. Wild type and the hy2 pubs double mutant were used to dissect the regulated genes of moss phytochromes. For details, please see PMID: . Overall design: 4 samples, dark-grown wild-type and pubs hy2 protonema as time 0 control, followed by red light irradiation for one hour respectively

红光/远红光光受体光敏色素（phytochrome）可介导植物的光形态建成响应。为实现光感知与信号转导，光敏色素需与开链四吡咯分子结合作为生色团。四吡咯生色团的生物合成依赖铁氧还蛋白依赖的胆色素还原酶（ferredoxin-dependent bilin reductases，FDBRs）家族成员。小立碗藓（Physcomitrella patens）中存在两种FDBRs：HY2与PUBS。同时敲除这两个基因即可获得光敏色素缺陷突变体。本数据集提供了黑暗培养并经1小时红光照射的小立碗藓原丝体的转录组谱数据。实验选用野生型与hy2 pubs双突变体材料，以剖析小立碗藓光敏色素调控的靶基因。详细信息请参见PMID: 。实验设计概述：共设置4个样本，分别为黑暗培养的野生型及hy2 pubs双突变体原丝体作为0时刻对照，随后分别对两组样本施加1小时红光照射。