Expression data of 3 colorectal carcinoma cell lines with or without exposure to radiation

To explore long non-coding RNA (lncRNA) associated with radiosensitivity in colorectal carcinoma cell lines, expression profiles of Lovo, RKO and SW480 cell lines with different levels of radiosensitivity were investigated on ArrayStar platform. A probe-remapping strategy was applied to aquire the accurate probe annotation. By comparing expression profiles of cells with exposure to radiation with those of control sample (cells without exposure), a list of lncRNAs associated with radiation response was compiled. GSEA was then performed to explore their biological functions. A six chip study using total RNA collected from three cell lines (Lovo, RKO and SW480) with or without exposure to 2Gy dose of radiation.

为探究结直肠癌细胞系中与放射敏感性相关的长链非编码RNA（long non-coding RNA, lncRNA），本研究在ArrayStar平台上对不同放射敏感性水平的Lovo、RKO及SW480细胞系的表达谱进行了检测。研究采用探针重映射策略以获取精准的探针注释信息。通过对比受辐照细胞与对照样本（未受辐照的细胞）的表达谱，本研究筛选得到一系列与辐射应答相关的lncRNA。随后通过基因集富集分析（Gene Set Enrichment Analysis, GSEA）探究这些lncRNA的生物学功能。本研究为一项包含6张芯片的实验，所用总RNA提取自经2Gy剂量辐照或未辐照的3种细胞系（Lovo、RKO及SW480）。