Comparison of normal breast epithelium and stroma from reduction and I.D.C. cases. Homo sapiens

Histologically normal breast epithelium and stroma were laser capture microdissected from breast reduction specimens and from specimens of invasive ductal carcinoma. The objective of the study was to compare normal reduction tissues to tissues adjacent to I.D.C. to determine whether adjacent normal tissues contained expression profiles correlated with characteristics of the primary tumor and to identify markers of normal epithelium and stroma. Keywords: disease state analysis Overall design: common reference design. 66 samples replicated twice as dye swaps generating 132 arrays.

本研究从乳房缩小成形术标本及浸润性导管癌（invasive ductal carcinoma, IDC）标本中，获取经激光捕获显微切割（Laser Capture Microdissection, LCM）的组织学正常乳腺上皮与间质。本研究旨在对比正常乳房缩小成形术组织与浸润性导管癌旁组织，以明确癌旁正常组织是否存在与原发肿瘤特征相关的基因表达谱，并筛选正常乳腺上皮及间质的生物标志物。关键词：疾病状态分析。实验设计：采用通用参照设计。共纳入66份样本，经染料互换重复实验两次，最终获得132张基因芯片阵列。