Global gene expression analysis of pluripotent cell lines and corresponding starting donor source cells

Global gene expression analysis of induced pluripotent stem cell lines and their corresponding source cells Total RNA was harvested from H9 hESC (P51), non-integrated episomal CB-iPSC clones 6.2, 6.11, 6.13, (P14), 19.11, (P11), nonviral KER-iPSC clones KA.1, KA.3 (P13) nonviral FFB-iPSC: F.1, F.6 (P14) and viral fibroblast iPSC clones IMR1 (P66), IMR4 (P64). A single sample of each condition was used for this analysis.诱导多能干细胞（induced pluripotent stem cell, iPSC）系及其对应来源细胞的全基因表达分析。本研究从以下样本中提取总RNA：H9人胚胎干细胞（human embryonic stem cell, hESC，第51代）、非整合型附加体载体构建的脐带血诱导多能干细胞（cord blood-induced pluripotent stem cell, CB-iPSC）克隆6.2、6.11、6.13（第14代）、19.11（第11代）、非病毒载体构建的角质形成细胞诱导多能干细胞（keratinocyte-induced pluripotent stem cell, KER-iPSC）克隆KA.1、KA.3（第13代）、非病毒载体构建的成纤维细胞诱导多能干细胞（fibroblast-induced pluripotent stem cell, FFB-iPSC）克隆F.1、F.6（第14代），以及病毒载体构建的成纤维细胞诱导多能干细胞克隆IMR1（第66代）、IMR4（第64代）。本分析仅采用每种实验条件下的单一样本进行。