Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A

Bacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids, agar, and yeast extract. The fluorescent dye specifically attaches to cellulose, enabling bacterial colonies to be observed under ultraviolet light. The study employed YESCA medium supplemented with calcofluor-white, as well as YESCA + 0.2% glucose medium supplemented with calcofluor-white.
The dataset contains original photos of bacterial colonies of the following E. coli strains: BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP AAEC191A/pCC90, AAEC191A/pACYCpBAD, AAEC191A/pCC90 Dra D-mut, AAEC191A/pCC90 D54-STOP, BL21(DE3), AAEC191A and CFT073. Bacteria were cultured in YESCA medium with the addition of calcofluor-white at a temperature of 25OC and 37OC  for 5 days. Photographs of the colony were taken at one-day intervals.

形成生物膜的细菌可产生胞外基质（extracellular matrix，ECM），其中纤维素为其核心组成成分。一种用于检测微生物纤维素合成能力的方法为菌落钙荧光白（calcofluor white）染色法：将荧光染料钙荧光白添加至由酪蛋白氨基酸、琼脂及酵母提取物配制的稳定YESCA培养基中，该染料可特异性结合纤维素，使细菌菌落能够在紫外线下被观测到。本研究采用了添加钙荧光白的YESCA培养基，以及添加钙荧光白的含0.2%葡萄糖的YESCA培养基。

本数据集包含以下大肠杆菌（Escherichia coli，E. coli）菌株的菌落原始照片：BL21(DE3)/pCC90、BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra D-mut、BL21(DE3)/pCC90 D54-STOP AAEC191A/pCC90、AAEC191A/pACYCpBAD、AAEC191A/pCC90 Dra D-mut、AAEC191A/pCC90 D54-STOP、BL21(DE3)、AAEC191A及CFT073。细菌于添加钙荧光白的YESCA培养基中，分别在25℃与37℃下培养5天，且每隔1天拍摄一次菌落照片。