Determination of ErfA regulon by RNA-seq in IHMA87 and PAO1

ErfA is a transcription factor of Pseudomonas aeruginosa. We here define the genes impacted by the deletion of erfA by RNA-seq. Overall design: RNA-seq analysis of WT vs erfA mutant, in both P. aeruginosa PAO1 and IHMA87 in LB liquid culture at OD=1, in duplicates.

ErfA是铜绿假单胞菌（Pseudomonas aeruginosa）的转录因子。本研究通过RNA测序（RNA-seq）明确了ErfA基因缺失所影响的全部基因。实验整体设计：在LB液体培养基中培养至光密度（OD）=1时，分别对铜绿假单胞菌PAO1与IHMA87菌株的野生型（WT）和ErfA缺失突变株进行RNA-seq分析，每组设置两次生物学重复。