mRNA expression profiles in androgenetic, parthenogenetic and fertilized embryonic stem cells. Mus musculus

In this study, mRNA expression profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from androgenetic (aESC), parthenogenetic (pESC) and fertilized (fESC) blastocysts. Results showed that 2394, 87 and 1788 mRNAs were differentially expressed in the aESCs vs. fESCs, pESCs vs. fESCs and aESCs vs. pESCs, respectively. Overall design: Androgenetic, parthenogenetic and fertilized embryonic stem cell lines were established from androgenetic, parthenogenetically activated and fertilized blasotocyst. mRNA microarrays were repeated three times using passages 6, 7 and 8.

本研究通过Illumina微阵列（Illumina microarray）技术，对源自孤雄生殖胚胎干细胞（androgenetic embryonic stem cells, aESC）、孤雌生殖胚胎干细胞（parthenogenetic embryonic stem cells, pESC）及正常受精胚胎干细胞（fertilized embryonic stem cells, fESC）囊胚的小鼠胚胎干细胞（embryonic stem cells, ESCs）的mRNA表达谱进行了检测分析。结果显示，在孤雄胚胎干细胞与正常受精胚胎干细胞、孤雌胚胎干细胞与正常受精胚胎干细胞、孤雄胚胎干细胞与孤雌胚胎干细胞的对比组中，分别存在2394、87及1788个差异表达mRNA。
实验整体设计：分别从孤雄生殖激活、孤雌生殖激活及正常受精来源的囊胚中构建孤雄、孤雌及正常受精胚胎干细胞系；采用第6、7、8代细胞开展三次重复mRNA微阵列实验。