Analysis of gene expression in human malignant rhabdoid cells following prolonged treatment with the histone deacetylase inhibitor, Panobinostat

We hypothesized that prolonged treatment of malignant rhabdoid tumor cells with low-dose HDACi will drive cellular differentiation. We assessed changes in gene expression following 21 days treatment with the HDACi, Panobinostat, versus a DMSO vehcile control in three human MRT cell lines. Total RNA obtained from three human malignant rhabdoid tumor cell lines (G401, SJSC, STM91-01) cultured for 21 days in the presence of low-dose Panobinostat was compared to 21-day treated DMSO control cells. All treatments and cell lines were performed in triplicate.

我们提出假说：低剂量组蛋白去乙酰化酶抑制剂（HDACi）长期处理恶性横纹肌样瘤细胞可诱导细胞分化。本研究针对三种人恶性横纹肌样瘤细胞系，对比分析了经帕比司他（Panobinostat）处理21天与二甲基亚砜（DMSO）载体对照处理后的基因表达变化。我们将在低剂量帕比司他环境中培养21天的三种人恶性横纹肌样瘤细胞系（G401、SJSC、STM91-01）所提取的总RNA，与经21天DMSO处理的对照细胞进行了比较。所有处理组及细胞系实验均设置三次生物学重复。