Comprehensive genome annotation of the model ciliate Tetrahymena thermophila by in-depth epigenetic and transcriptomic profiling. Tetrahymena thermophila strain:SB210

To comprehensively annotate genes of Tetrahymena macronuclear genome, we collected extensive transcriptomic data spanning various cell stages. In addition, to better ascertain the transcripts orientation and the transcription start/end sites, we incorporated strand-specific transcriptomic data and epigenetic data including the histone modification H3 lysine 4 tri-methylation (H3K4me3), the histone variant H2A.Z, nucleosome positioning, N6-methyldeoxyadenine (6mA), and chromatin accessibility.

为全面注释四膜虫（Tetrahymena）大核基因组的基因，我们收集了覆盖不同细胞时期的大规模转录组数据。此外，为更精准地确定转录本方向以及转录起始/终止位点，我们整合了链特异性转录组数据，以及组蛋白H3赖氨酸4三甲基化（H3K4me3）、组蛋白变体H2A.Z、核小体定位、N6-甲基脱氧腺嘌呤（6mA）和染色质开放性等表观遗传学数据。