RNA extracted from Sf21 cell line were sequenced and used in genome annotation worflow.. Sf21 RNAseq reads

The transcriptomics reads were used to build a new gene prediction workflow Using this approach, we could predict 21,506 Sf21 genes which were then functionally annotated. This integrative approach could be in theory applied to any genomes and results to valuable resources. With these information available, Sf21 cells will become an even better tools for protein expression and could be used in a wide range of applications, from promoter identifications to genome engineering and editing.

本研究利用转录组读段（transcriptomics reads）构建了全新的基因预测流程。借助该流程，我们共预测得到21506个 Sf21 细胞的基因，并对这些基因完成了功能注释。该整合式策略理论上可应用于任意基因组的分析，并可产出极具价值的研究资源。依托上述研究成果，Sf21细胞将成为更为优质的蛋白表达宿主工具，可广泛应用于从启动子鉴定到基因组工程与编辑的各类研究场景。