Additional file 5: of Metabolic flux analysis for metabolome data validation of naturally xylose-fermenting yeasts

a Correlation (R2) between calculated and measured fluxes - S. stipitis. Acetyl-CoA (ACCOA), dihydroxy-acetone-phosphate (DHAP), erythrose-4-phosphate (E4P), fructose-6-phosphate (F6P), glucose-6-phosphate (G6P), malate (MAL), phosphoenolpyruvate (PEP), pyruvate (PEP), ribose-5-phosphate (R5P), ribulose-5-phosphate (RU5P), and sedoheptulose-7-phosphate (S7P) were the metabolites measured. (X-axis) show the calculated fluxes using the constrained values of products formation. (Y-axis) show measured fluxes with respectively metabolites concentrations. Graphics in square presents a correlation higher than 0.9. Flux rates are in mmol/gCDW.h− 1. b Correlation (R2) between calculated and measured fluxes - S. arborariae. Acetyl-CoA (ACCOA), dihydroxy-acetone-phosphate (DHAP), erythrose-4-phosphate (E4P), fructose-6-phosphate (F6P), glucose-6-phosphate (G6P), malate (MAL), phosphoenolpyruvate (PEP), pyruvate (PYR), ribose-5-phosphate (R5P), ribulose-5-phosphate (RU5P), and sedoheptulose-7-phosphate (S7P) were the metabolites measured. (X-axis) show the calculated fluxes using the constrained values of products formation. (Y-axis) show measured fluxes with respectively metabolites concentrations. Graphics in square presents a correlation higher than 0.9. Flux rates are in mmol/gCDW.h− 1. c Correlation (R2) between calculated and measured fluxes - S. passalidarum. Acetyl-CoA (ACCOA), dihydroxy-acetone-phosphate (DHAP), erythrose-4-phosphate (E4P), fructose-6-phosphate (F6P), glucose-6-phosphate (G6P), malate (MAL), phosphoenolpyruvate (PEP), pyruvate (PYR), ribose-5-phosphate (R5P), ribulose-5-phosphate (RU5P), and sedoheptulose-7-phosphate (S7P) were the metabolites measured. (X-axis) show the calculated fluxes using the constrained values of products formation. (Y-axis) show measured fluxes with respectively metabolites concentrations. Graphics in square presents a correlation higher than 0.9. Flux rates are in mmol/gCDW.h− 1. (ZIP 183 kb)

a 树干毕赤酵母（S. stipitis）计算通量与实测通量的决定系数（R²）相关性分析。本次检测的代谢物包括：乙酰辅酶A（Acetyl-CoA, ACCOA）、二羟丙酮磷酸（dihydroxy-acetone-phosphate, DHAP）、赤藓糖-4-磷酸（erythrose-4-phosphate, E4P）、果糖-6-磷酸（fructose-6-phosphate, F6P）、葡萄糖-6-磷酸（glucose-6-phosphate, G6P）、苹果酸（malate, MAL）、磷酸烯醇式丙酮酸（phosphoenolpyruvate, PEP）、丙酮酸（pyruvate, PEP）、核糖-5-磷酸（ribose-5-phosphate, R5P）、核酮糖-5-磷酸（ribulose-5-phosphate, RU5P）以及景天庚酮糖-7-磷酸（sedoheptulose-7-phosphate, S7P）。图中X轴代表基于产物形成约束值计算得到的通量，Y轴代表结合对应代谢物浓度的实测通量。方形标注区域内的图形相关系数高于0.9。通量速率单位为毫摩尔每克细胞干重每小时（mmol/gCDW·h⁻¹）。
b 树状毕赤酵母（S. arborariae）计算通量与实测通量的决定系数（R²）相关性分析。本次检测的代谢物包括：乙酰辅酶A（Acetyl-CoA, ACCOA）、二羟丙酮磷酸（dihydroxy-acetone-phosphate, DHAP）、赤藓糖-4-磷酸（erythrose-4-phosphate, E4P）、果糖-6-磷酸（fructose-6-phosphate, F6P）、葡萄糖-6-磷酸（glucose-6-phosphate, G6P）、苹果酸（malate, MAL）、磷酸烯醇式丙酮酸（phosphoenolpyruvate, PEP）、丙酮酸（pyruvate, PYR）、核糖-5-磷酸（ribose-5-phosphate, R5P）、核酮糖-5-磷酸（ribulose-5-phosphate, RU5P）以及景天庚酮糖-7-磷酸（sedoheptulose-7-phosphate, S7P）。图中X轴代表基于产物形成约束值计算得到的通量，Y轴代表结合对应代谢物浓度的实测通量。方形标注区域内的图形相关系数高于0.9。通量速率单位为毫摩尔每克细胞干重每小时（mmol/gCDW·h⁻¹）。
c 巴沙达毕赤酵母（S. passalidarum）计算通量与实测通量的决定系数（R²）相关性分析。本次检测的代谢物包括：乙酰辅酶A（Acetyl-CoA, ACCOA）、二羟丙酮磷酸（dihydroxy-acetone-phosphate, DHAP）、赤藓糖-4-磷酸（erythrose-4-phosphate, E4P）、果糖-6-磷酸（fructose-6-phosphate, F6P）、葡萄糖-6-磷酸（glucose-6-phosphate, G6P）、苹果酸（malate, MAL）、磷酸烯醇式丙酮酸（phosphoenolpyruvate, PEP）、丙酮酸（pyruvate, PYR）、核糖-5-磷酸（ribose-5-phosphate, R5P）、核酮糖-5-磷酸（ribulose-5-phosphate, RU5P）以及景天庚酮糖-7-磷酸（sedoheptulose-7-phosphate, S7P）。图中X轴代表基于产物形成约束值计算得到的通量，Y轴代表结合对应代谢物浓度的实测通量。方形标注区域内的图形相关系数高于0.9。通量速率单位为毫摩尔每克细胞干重每小时（mmol/gCDW·h⁻¹）。本数据集为183 KB的ZIP压缩包。