The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy. The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy

To reveal the involved gene regulatory pathways, we carried out RNA-seq transcriptomics analysis on the cells treated with or without PA, treated with PA+OA and PA+BMS. Overall design: con: HepaRG without treated for 4h. PA: HepaRG were treated with 400μM deuterium-labeled palmitate acid (PA) for 4h. PA+OA: HepaRG were treated with 400μM PA and 200μM oleic acid (OA) for 4h PA+BMS: HepaRG were treated with 50μM BMS-309403 (BMS) for 24 h during concomitant incubation with 400μM PA for 4 h.

为揭示所涉及的基因调控通路，本研究对经PA处理、未经PA处理，以及经PA+OA、PA+BMS处理的细胞开展了RNA测序转录组学分析。
实验设计概况：
对照组（con）：未经处理的HepaRG细胞，孵育时长为4小时。
PA组：将HepaRG细胞置于400μM氘标记棕榈酸（PA）中孵育4小时。
PA+OA组：将HepaRG细胞置于400μM PA与200μM油酸（OA）的混合体系中孵育4小时。
PA+BMS组：将HepaRG细胞与400μM PA共孵育4小时期间，同时用50μM BMS-309403（BMS）处理24小时。