mRNA expression analysis of radiation-induced skin fibrosis of rats

This study aims to compared mRNA expression between radiation-induced fibrotic skin and adjacent normal tissues of rats by RNA-Seq. Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas and irradietd areas were collected and subjected to mRNA expression analysis. Overall design: Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas and irradietd areas were collected and subjected to RNA sequencing.本研究旨在通过RNA测序（RNA-Seq）技术，对比大鼠放射性纤维化皮肤与其邻近正常皮肤组织的信使核糖核酸（mRNA）表达情况。选取4周龄的雄性斯普拉格-道利（Sprague-Dawley, SD）大鼠，使用6 MeV电子束加速器（Clinac 2100EX，瓦里安医疗系统股份有限公司，加利福尼亚州）以750 cGy/min的剂量率，单次向治疗区域施加45 Gy的辐射剂量。采集未照射区域与照射区域的皮肤组织，开展mRNA表达分析。实验整体设计：选取4周龄的雄性斯普拉格-道利（SD）大鼠，使用6 MeV电子束加速器（Clinac 2100EX，瓦里安医疗系统股份有限公司，加利福尼亚州）以750 cGy/min的剂量率，单次向治疗区域施加45 Gy的辐射剂量。采集未照射区域与照射区域的皮肤组织，进行RNA测序分析。