Expression data from E12.5 mouse embryonic heart. Mus musculus

We prepared RNA from mouse embryonic hearts and performed mRNA micro-array analysis to compare gene expression profiles between control and Rhau-deletion mice (E12.5), in order to understand how RHAU regulates heart development. Overall design: Three hearts were dissected from each group of control and Rhau-deletion mice and total RNA was isolated from these hearts. In total, six RNA samples were applied for microarray analysis (Affymetrix Mouse 430 2.0 Array, Shanghai Biotechnology Co. Ltd.).

我们从小鼠胚胎心脏中提取总RNA，开展mRNA微阵列分析，以对比对照组与Rhau基因敲除小鼠（胎龄E12.5）的基因表达谱，进而解析RHAU调控心脏发育的分子机制。
实验整体设计：分别从对照组与Rhau基因敲除小鼠组中各解剖3枚心脏，从上述心脏样本中提取总RNA。最终共制备6份RNA样本，采用Affymetrix Mouse 430 2.0 Array芯片进行微阵列分析，实验由上海生物科技有限公司（Shanghai Biotechnology Co. Ltd.）完成。