Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of <i>Pseudomonas aeruginosa</i> Isolated from Burn Wound Infections

Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections.生物膜（Biofilm）是铜绿假单胞菌（Pseudomonas aeruginosa）的重要毒力因子，在抗生素耐药性及慢性烧伤创面感染中发挥关键作用。当前亟需研发可降解烧伤创面生物膜、提升现有抗菌剂临床疗效的抗铜绿假单胞菌治疗剂。本研究评估了α-甘露糖苷酶（α-mannosidase、β-甘露糖苷酶（β-mannosidase及胰蛋白酶（trypsin）对铜绿假单胞菌生物膜的降解效果，以及其对头孢他啶（ceftazidime）最小生物膜清除浓度（minimum biofilm eliminating concentrations, MBEC）的降低作用。所有受试酶均能破坏生物膜，并降低头孢他啶最小生物膜清除浓度。然而，仅胰蛋白酶对A-431人表皮样癌细胞系无细胞毒性。综上，鉴于胰蛋白酶的性能优于甘露糖苷酶，其有望成为对抗铜绿假单胞菌烧伤创面感染的潜在治疗剂。