Expression and Prognostic impact of LncRNAs in Acute Myeloid Leukemia

Long noncoding RNAs (lncRNAs) are transcripts longer than 200 nucleotides located within the intergenic stretches or overlapping antisense transcripts of protein coding genes. LncRNAs are involved in numerous biological roles including imprinting, epigenetic regulation, apoptosis and cell-cycle. To determine whether lncRNAs are associated with clinical features and recurrent mutations in older patients (aged ≥60 years) with cytogenetically normal (CN) acute myeloid leukemia (AML), we evaluated lncRNA expression in 148 untreated older CN-AML cases using a custom microarray platform. In this study, we analyzed a large set of older CN-AML patients using custom lncRNA microarrays to investigate whether lncRNA expression is associated with clinical features, molecular abnormalities and outcome and to build a prognostic lncRNA signature that was subsequently validated using RNA sequencing. This submission represents RNA-Seq component of study.

长链非编码RNA（Long noncoding RNAs, lncRNAs）是一类长度超过200个核苷酸的转录本，可定位于基因间区，或与蛋白编码基因的重叠反义转录本发生重叠。长链非编码RNA参与诸多生物学功能，包括基因组印记、表观遗传调控、细胞凋亡及细胞周期进程。为明确长链非编码RNA是否与年龄≥60岁的细胞遗传学正常（cytogenetically normal, CN）急性髓系白血病（acute myeloid leukemia, AML）老年患者的临床特征及复发性突变相关，本研究采用定制微阵列平台，对148例未经治疗的老年CN-AML患者的lncRNA表达谱进行了检测。本研究通过定制lncRNA微阵列，对大型老年CN-AML患者队列展开分析，以探究lncRNA表达与临床特征、分子异常及预后结局的关联，并构建预后相关lncRNA特征，后续通过RNA测序对该特征予以验证。本提交数据即为该研究的RNA-Seq组分。