Additional file 2 of Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Additional file 2: Table S1: Detailed list of species and samples, together with DNA extraction and long-read sequencing results of each SMRT cell. Table S2: Assembly statistics for Cathorops nuchalis and Cathorops wayuu. Table S3: High levels of contamination in samples of Russian desman, Hazel dormouse and slow worm. Table S4: Bradypus torquatus genome assemblies generated with data from ultra-low input libraries prepared with polymerases A/B and/or C at different coverages. Table S5: Genome assemblies generated with polymerases A/B and/or C data at different coverages for Elyisa timida, Scintilla philippinensis, and Podura aquatica. Table S6: Assembly statistics for all tested contig assemblers using sequenced PacBio HiFi reads from four SMRTs produced with polymerase A/B and one SMRT produced with polymerase C for Bradypus torquatus.附加文件2：表S1：物种与样本详细清单，连同每个单分子实时（SMRT）测序单元的DNA提取与长读长测序结果。表S2：裸项海鲶（Cathorops nuchalis）与瓦尤海鲶（Cathorops wayuu）的基因组组装统计结果。表S3：俄罗斯麝鼹（Russian desman）、榛睡鼠（Hazel dormouse）以及慢缺肢蜥（slow worm）的样本中存在高水平污染。表S4：使用聚合酶A/B和/或C制备的超低起始量文库数据，在不同测序覆盖度下生成的鬃毛三趾树懒（Bradypus torquatus）基因组组装结果。表S5：针对Elyisa timida、菲律宾闪螺（Scintilla philippinensis）以及水圆跳虫（Podura aquatica），使用聚合酶A/B和/或C的数据在不同测序覆盖度下生成的基因组组装结果。表S6：针对鬃毛三趾树懒（Bradypus torquatus），使用聚合酶A/B制备的4个SMRT测序单元以及聚合酶C制备的1个SMRT测序单元产出的PacBio HiFi测序读段，所有经测试的序列重叠群组装工具的组装统计结果。