Nano-TRAP Input and IP sequencing of WT and Rpl3l-/- mouse hearts. Nano-TRAP Input and IP sequencing of WT and Rpl3l-/- mouse hearts

RPL3L is a ribosomal protein expressed exclusively in adult straited muscle tissues, and a paralogue of the ubiquitously expressed RPL3. Here, we are looking into the effects of Rpl3l knockout on translation in adult mice hearts. Overall design: Nano-TRAP (Input and IP) done on WT and Rpl3l-/- mouse hearts in biological triplicates.

RPL3L是一种仅在成体横纹肌组织中特异性表达的核糖体蛋白，亦是普遍表达的RPL3的旁系同源基因。本研究旨在探究Rpl3l基因敲除对成年小鼠心脏翻译进程的影响。整体实验设计：针对野生型（Wild Type, WT）与Rpl3l基因敲除（Rpl3l-/-）小鼠心脏，各设置三份生物学重复，开展包含Input组分与免疫沉淀（Immunoprecipitation, IP）组分的Nano-TRAP实验。