SM003 - icSARS CoV, SARS MA15 wild type and SARS BatSRBD mutant virus infections of C57BL6 mice - A time course

This purpose of this experiment was to investigate the transcriptional differences between mice infected with icSARS CoV, SARS MA15 wild type or SARS BatSRBD viruses. Overview of Experiment: Groups of 20 week old C57BL6 mice were infected with icSARS CoV, Wild Type SARS MA15 or SARS BatSRBD mutant viruses. Infections were done at 10^4 PFU or 10^5 PFU or time-matched mock infected. Time points were 1, 2, 4 and 7 d.p.i. There were 3-5 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

本实验旨在探究感染icSARS-CoV、SARS MA15野生型病毒或SARS BatSRBD病毒的小鼠之间的转录组差异。
实验概述：将20周龄的C57BL/6小鼠分为多组，分别以10^4噬斑形成单位（PFU）或10^5 PFU的剂量感染icSARS-CoV、SARS MA15野生型病毒或SARS BatSRBD病毒，同时设置与感染组时间匹配的模拟感染对照组。实验采集的时间点为感染后1、2、4、7天（d.p.i.），每个剂量-时间点组包含3~5只小鼠。研究人员收集肺组织样本，用于病毒载量、转录组及蛋白质组学分析，并同步监测小鼠的体重变化与存活率。