Transcriptomic change in response to high ambient temperature in two Arabidopsis thaliana ecotypes. Arabidopsis thaliana

To observe transcriptomic change in response to high ambient temperature in two Arabidopsis thaliana ecotypes: Col-0 and Sij-4. One hundred 6-day-old seedlings of Col-0 or Sij-4 grown at 23 ºC or 27 ºC in growth chambers (GR-36, Percival Scientific, Canada) in SD were harvested around 1.00 PM (5 hours after the beginning of the light regime). The RNA was extracted using Isolate II RNA plant kit (Bioline Pty Ltd, Australia) and RNASeq was done with Illumina HiSeq2000 platform by BGI (BGI, China). Three and two biological replicates were used for Col-0 and the Sij-4 samples respectively.

本数据集旨在探究两种拟南芥（Arabidopsis thaliana）生态型Col-0与Sij-4在响应环境高温时的转录组学（transcriptomic）变化。将在23℃或27℃、生长箱（GR-36，加拿大Percival Scientific公司）中、短日照（Short Day，SD）条件下培养6天的Col-0与Sij-4幼苗各100株，于光照时段开始后5小时（约下午1:00）取样收获；采用Isolate II RNA植物提取试剂盒（澳大利亚Bioline Pty Ltd公司）提取总RNA，由中国华大基因（BGI）依托Illumina HiSeq2000平台完成RNA测序（RNA Sequencing，RNASeq）；其中Col-0样本设置3次生物学重复，Sij-4样本设置2次生物学重复。