The Israeli BAG

This Summary will only portray a very limited scope of the BAG proposal due to its size and more information will be available in the Global Summary. We plan establish a comprehensive structural narrative for the assembly and entry of viruses from the Bunyavirales family using X-ray crystallography, biophysics, biochemistry, and molecular virology. Using X-ray crystallography to obtain the 3D structure of GN and GC in different conformations and from different bunyavituses. We will pursue a structure of GN or GC bound to a neutralizing antibody to obtain new data for vaccine design. In the SAXS arena, we plan to continue the study on MT (microtubules) and expand the experiments with different nucleotide type and concentrations, (GTP, GDP, GMPCPP, and GMPPCP, GTP-gammaS). We will perform at selected points SEC-SAXS experiments in the presence of full-length tau (4RL). Our preliminary data revealed that 4RL promote MT assembly at 36C and of single tubulin rings at low temperatures.

鉴于本提案篇幅限制，本摘要仅涵盖BAG提案的极小一部分内容，完整信息可查阅全局摘要。我们计划依托X射线晶体学、生物物理学、生物化学与分子病毒学技术手段，为布尼亚病毒目（Bunyavirales）病毒的组装与入侵过程构建完整的结构生物学阐释体系。我们将通过X射线晶体学解析不同构象下、以及不同布尼亚病毒株中GN与GC蛋白的三维结构。我们还将解析GN或GC与中和抗体结合的复合物结构，为疫苗设计提供全新的实验数据。在小角X射线散射（SAXS）研究方向上，我们计划延续针对微管（MT）的相关研究，并拓展不同核苷酸类型与浓度条件下的实验，涉及的核苷酸包括GTP、GDP、GMPCPP、GMPPCP及GTP-γS。我们还将在选定的实验节点开展添加全长tau蛋白（4RL）的尺寸排阻色谱-小角X射线散射（SEC-SAXS）实验。我们的初步实验数据显示，4RL可在36摄氏度条件下促进微管组装，并在低温环境下促成单微管环的形成。