Gene expression signatures for plumbagin (PL) treatment in OSCC cell lines. Gene expression signatures for plumbagin (PL) treatment in OSCC cell lines

To investigate the effect of PL on gene expression, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to suppress the cell proliferation of OSCC cell lines. SAS and HSC-3 cells were treated with PL (5 μmol/L) for 4 h in vitro. Overall design: SAS and HSC-3 cells were treated with PL for 4 h in vitro.

为探究PL对基因表达的影响，本研究采用全基因组微阵列表达谱分析作为发现平台，以鉴定具备抑制口腔鳞状细胞癌（Oral Squamous Cell Carcinoma，OSCC）细胞系增殖潜力的基因。体外实验中，将SAS细胞与HSC-3细胞以浓度为5 μmol/L的PL处理4小时。
实验整体设计：体外将SAS细胞与HSC-3细胞以PL处理4小时。