Pitfalls in Single Clone CRISPR-Cas9 Mutagenesis to Fine-map Regulatory Intervals

We report gene expression of four and three single-cell derived clones from HL60 and HL60/S4 cell lines, respectively, and the gene expression of bulk parental lines. We also report gene expression of control and mnocyte-derived HL60 (n=2, n=2, respectively) and HL60/S4 (n=2, n=2, respectively) single-cell clones, and control and neutrophil-derived HL60 (n=4, n=4, respectively) and HL60/S4 (n=4, n=4, respectively) single-cell clones. Single-cell clones were derived from HL60 and HL60/S4 cell lines. The transcriptome of parental clones and bulk cells was profiled by RNA-Seq. Those single-cell derived clones were then differentiated into neutrophils and monocytes. The gene expression of vehicle controls and differentiated clones was profiled by RNA-Sequencing. Each clone with the same type of treatment or control has two replicates.

本研究报道了分别源自HL60与HL60/S4细胞系的4株和3株单细胞衍生克隆的基因表达情况，同时提供了批量亲本细胞系的基因表达数据。此外，本研究还涵盖了对照组、单核细胞来源的HL60单细胞克隆（每组样本量n=2），以及对照组、单核细胞来源的HL60/S4单细胞克隆（每组样本量n=2）的基因表达数据；同时包含对照组、中性粒细胞来源的HL60单细胞克隆（每组样本量n=4），以及对照组、中性粒细胞来源的HL60/S4单细胞克隆（每组样本量n=4）的基因表达数据。上述所有单细胞克隆均由HL60与HL60/S4细胞系分离衍生得到。研究人员通过RNA测序（RNA-Sequencing，RNA-Seq）对亲本克隆与批量细胞的转录组进行了测序分析。随后将上述单细胞衍生克隆分别诱导分化为中性粒细胞与单核细胞，并通过RNA测序对溶剂对照组细胞与分化后的克隆的基因表达水平进行了检测。每一种处理组或对照组的克隆均设置2次生物学重复。