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Additional file 1: Figure S1. of Transcriptomic profiling of Burkholderia phymatum STM815, Cupriavidus taiwanensis LMG19424 and Rhizobium mesoamericanum STM3625 in response to Mimosa pudica root exudates illuminates the molecular basis of their nodulation competitiveness and symbiotic evolutionary history

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Venn diagram of gene orthologs across the 3 bacteria. Figure S2. Symbiotic plasmids syntenies and differentially regulated genes. Figure S3. Summary of RNAseq results per replicate and condition. Figure S4. Comparison of pVal and Fold Change values on the number of DEG. Figure S5. Genes targeted 5 for qPCR (A) & primers used (B). Figure S6. Comparison of gene expression between RNAseq counts and qPCR. Table S1. Excel tables of all DEG data shared or specific. Figure S8. Myo-inositol catabolism operons in BP, RM and CT. Figure S9. Biolog GN20 API galleries results for BP, CT and RM. Figure S10. Annotation of the putative rhizobitoxine biosynthesis operon in BP. Figure S11. Up-regulated (A) and down-regulated (B) T6SS in B. phymatum (BP). Figure S12. T4SS of B. phymatum STM815. Figure S13. Elution profiles of C. taiwanensis RNA samples. Figure S14. Scatter plots (A) & Volcano plot (B) of RNAseq data. (ZIP 15222 kb)

3种细菌间基因直系同源物的维恩图。 图S2:共生质粒共线性与差异调控基因。 图S3:各生物学重复与处理条件下的RNA测序(RNAseq)结果汇总。 图S4:差异表达基因(differentially expressed gene, DEG)数量与p值、倍数变化(Fold Change)值的比较。 图S5:用于定量聚合酶链反应(quantitative PCR, qPCR)的5个靶标基因(A)及所用引物(B)。 图S6:RNA测序计数与定量聚合酶链反应(qPCR)的基因表达量比较。 表S1:所有共享或特异性差异表达基因数据的Excel表格。 图S8:BP、RM与CT菌株中的肌醇(Myo-inositol)分解代谢操纵子。 图S9:BP、CT与RM菌株的Biolog GN20 API检测板结果。 图S10:BP菌株中推定根瘤菌毒素(rhizobitoxine)生物合成操纵子的注释信息。 图S11:食酸伯克霍尔德菌(B. phymatum, BP)中上调(A)与下调(B)的六型分泌系统(Type VI Secretion System, T6SS)。 图S12:食酸伯克霍尔德菌STM815的四型分泌系统(Type IV Secretion System, T4SS)。 图S13:台湾伯克霍尔德菌(C. taiwanensis)RNA样品的洗脱曲线。 图S14:RNA测序(RNAseq)数据的散点图(A)与火山图(Volcano plot)(B)。(压缩包大小15222 KB)
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2018-01-30
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