The effects of early systemic neuroinflammation on the brain's response to further inflammatory insults

To identify how an early life first neuroinflammatory challenge affects response to subsequent challenges. Overall design: We estabilished a neontal two-hit model: for the first hit, mice were injected subcutaneously at P3 with 5mg/kg lipopolysaccharides (L) or saline (S);for the second hit, mice received 5mg/kg LPS (L) or saline (S) intraperitoneally. Animals were divided into four groups: SS, SL, LS, LL. Mice were sacrificed at P15, 24 hours after the second hit. RNA was extracted from the hippocampus for sequencing .

为探究生命早期首次神经炎症打击对机体后续刺激应答反应的影响。
实验整体设计：本研究构建了新生小鼠双打击模型：首次打击阶段，于小鼠出生后第3天（P3）皮下注射5mg/kg脂多糖（lipopolysaccharides，简称LPS，本文中以缩写L代指）或生理盐水（本文中以缩写S代指）；第二次打击阶段，通过腹腔注射给予5mg/kg LPS（即L）或生理盐水（S）。实验动物共分为四组：SS、SL、LS及LL组。于第二次打击后24小时，即小鼠出生后第15天（P15）处死所有小鼠，提取海马组织RNA进行测序。