Lipopolysaccharide effects on activation and apoptosis of primordial ovarian follicles in heifers

ABSTRACT The objective of the present study was to evaluate the effect of lipopolysaccharide (LPS) administration on activation and apoptosis of primordial follicles. There was no difference in the total number of follicles as well as in the different types of follicles. Furthermore, the LPS challenge didn’t modulate the expression of genes related with ovarian reserve (HAM), oocyte survival (Survivin), activation rate (Pten, KIT, KITL1, KITL2, AKT1, SIRT1), and follicular abnormalities. Therefore, the LPS exposure with 24h interval had no effect on activation rate and primordial follicles abnormalities, and also had no effect on expression of anti-apoptotic genes and genes related with ovarian reserve, oocyte survival, activation rate, and primordial follicles abnormalities.

摘要 本研究旨在评估脂多糖（lipopolysaccharide, LPS）给药对原始卵泡（primordial follicles）激活与细胞凋亡的影响。研究结果显示，卵泡总数及各类卵泡的数量均无差异。此外，脂多糖刺激并未调控与卵巢储备（ovarian reserve）、卵母细胞存活（oocyte survival）及激活率相关的基因表达，涉及的基因包括HAM、Survivin、Pten、KIT、KITL1、KITL2、AKT1与SIRT1，以及卵泡异常相关基因。综上，间隔24小时的脂多糖暴露既未对原始卵泡的激活率与异常情况产生影响，也未对抗凋亡基因及与卵巢储备、卵母细胞存活、激活率、原始卵泡异常相关的基因表达产生作用。