Next Generation Sequencing Facilitates Quantitative Analysis of Renca and B16 Murine Cancer Line Transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of Renca and B16 Murine Cancer Line Transcriptomes

Renca, a murine renal cell carcinoma, and B16F10, a murine melanoma, were transcriptionally profiled in a study designed to accompany an in vivo CRISPR screen to identify novel immunotherapy dependencies. Overall design: RNA sequencing libraries were generated from in vitro cultures of Renca and B16 cells.

本研究为配套一项旨在鉴定新型免疫治疗依赖靶点的体内CRISPR筛选实验，对小鼠肾细胞癌细胞系Renca与小鼠黑色素瘤细胞系B16F10开展了转录组测序分析。实验设计概况：从Renca细胞与B16细胞的体外培养物中构建RNA测序（RNA sequencing）文库。