SWATH-MS Analysis of Striatal Synaptic Mitochondria from Pink1 KO rats

This study investigates the proteomic alterations in striatal synaptic mitochondria isolated from 3-month-old wild-type and Pink1 KO rats using the SWATH-MS strategy. This dataset consists of 32 raw MS files, comprising 8 DIA (SWATH) and 24 DDA runs on a TripleTOF 5600 (SCIEX). Our findings revealed synaptic mitochondrial proteomic changes due to loss of Pink1.

本研究采用SWATH-MS（Sequential Window Acquisition of All Theoretical Mass Spectra）策略，对从3月龄野生型及Pink1敲除（Pink1 knockout, KO）大鼠中分离的纹状体突触线粒体的蛋白质组学改变开展探究。本数据集包含32份原始质谱文件，均在SCIEX公司的TripleTOF 5600质谱仪上完成测试，其中涵盖8次DIA（data-independent acquisition，SWATH为其典型模式）采集实验与24次DDA（data-dependent acquisition）采集实验。本研究结果揭示了Pink1基因缺失所引发的突触线粒体蛋白质组学变化。