m6A-mRNA&lncRNA Epitranscriptomic Microarray analysis for human liver tissues of healthy, HBV-infected with and without cirrhosis donors.

The samples of human liver tissues from the healthy donors, the HBV-infected patients without cirrhosis, and the HBV-infected patients with cirrhosis were collected. The tissues were then analyzed by the m6A-mRNA&lncRNA Epitranscriptomic miroarray. The Goal of this experiment was to determine the different of gene expression and m6a methylation of liver tissues among the healthy donors, the HBV-infected patients with and without cirrhosis. a total of 9 liver tissues from different donors were collected, including 3 from healthy donors, 3 from HBV-infected patients without cirrohsis, and 3 from HBV-infected patients with cirrhosis. The total RNA were extracted from the tissues within 24h at 4 °C and ahead of microarray analysis. Ethical approval for this study was provided by the Ethical Committee of the Second Affiliated Hospital of Air Force Military Medical University, Xi’an, China on 24 February 2016. All research was conducted in accordance with both the Declarations of Helsinki and Istanbul. Written consent was given in writing by all subjects.

本研究采集了健康供者、未合并肝硬化的乙型肝炎病毒（Hepatitis B Virus, HBV）感染者以及合并肝硬化的HBV感染者的人肝组织样本。随后采用m6A-mRNA&lncRNA表观转录组微阵列（m6A-mRNA&lncRNA Epitranscriptomic microarray）对上述组织开展检测分析。本实验的核心目标为探究健康供者、伴或不伴肝硬化的HBV感染者肝组织的基因表达与m6A甲基化水平差异。本研究共纳入9份来自不同供者的肝组织样本，其中健康供者、未合并肝硬化的HBV感染者、合并肝硬化的HBV感染者各3份。所有组织均在4℃环境下、微阵列分析前的24小时内完成总RNA提取。本研究的伦理审查已通过中国西安空军军医大学第二附属医院伦理委员会于2016年2月24日的审批。所有研究工作均严格遵循《赫尔辛基宣言》与《伊斯坦布尔宣言》的伦理规范。所有受试者均已签署书面知情同意书。