Data from: Two-current choice flumes for testing avoidance and preference in aquatic animals

Aquatic chemical ecology is an important and growing field of research that involves understanding how organisms perceive and respond to chemical cues in their environment. Research assessing the preference or avoidance of a water source containing specific chemical cues has increased in popularity in recent years, and a variety of methods have been described in the scientific literature. Two-current choice flumes have seen the greatest increase in popularity, perhaps because of their potential to address the broadest range of research questions.
Here, we review the literature on two-current choice flumes and show that there is a clear absence of standardized methodologies that make comparisons across studies difficult. Some of the main issues include turbulent flows that cause mixing of cues, inappropriate size of choice arenas for the animals, short experiments with stressed animals, failure to report how experiment and researcher biases were eliminated, general underreporting of methodological details, underutilization of collected data and inappropriate data analyses.
In this review, we present best practice guidelines on how to build, test and use two-current choice flumes to measure the behavioural responses of aquatic animals to chemical cues, and provide blueprints for flume construction. The guidelines include steps that can be taken to avoid problems commonly encountered when using two-current choice flumes and analysing the resulting data.
This review provides a set of standards that should be followed to ensure data quality, transparency and replicability in future studies in this field.

水生化学生态学（aquatic chemical ecology）是一门重要且不断发展的研究领域，旨在探究生物体如何感知并响应环境中的化学信号。近年来，评估生物体对含特定化学信号水源的偏好或规避行为的研究愈发受到关注，科学文献中也已记载了多种相关实验方法。双流选择水槽（two-current choice flumes）的应用增长最为显著，这或许是因为其能够应对最广泛的研究问题类型。
在此，我们对双流选择水槽相关的研究文献进行综述，发现目前明显缺乏标准化的实验方法，这使得不同研究之间的对比难以开展。当前存在的主要问题包括：湍流水流导致化学信号混合、受试动物的选择区域尺寸不匹配、实验时长过短且动物处于应激状态、未报告如何消除实验与研究者带来的偏倚、普遍未充分报告实验方法细节、收集的数据未得到充分利用，以及数据分析方法不当。
本综述针对如何搭建、调试并使用双流选择水槽以测量水生动物对化学信号的行为响应，提出了最佳实践指南，并提供了水槽搭建的设计蓝图。该指南涵盖了可用于规避双流选择水槽使用及后续数据分析过程中常见问题的具体步骤。
本综述提出了一套应遵循的标准，以确保该领域未来研究的数据质量、透明度与可重复性。