Human airway eptithelial cell subsets exhibit distinct transcriptional profiles in response to IFN-gamma derived from influenza specific CD8 T cells.

To evaluate the impact of IFN? secretion by influenza-specific CD8? T cells on human lung epithelial cells, we stimulated total primary human lung cells with influenza peptides in the presence or absence of an IFN?-blocking antibody. Following stimulation, epithelial cells (CD45?, CD31?, CD326?) were isolated by FACS and analyzed by single-cell RNA sequencing. Three stimulation conditions were compared including unstimulated, stimulated with influenza peptides plus isotype control antibody, and stimulated with influenza peptides plus anti-IFNg antibody. Overall design: scRNAseq of airway epithelial cells comparing three stimulation conditions. Total epithelial cells were divided into subsets based on key transcription factors post sequencing.

为评估流感特异性CD8α T细胞分泌的干扰素γ（IFNγ）对人肺上皮细胞的影响，本研究以流感肽刺激原代人肺总细胞，并设置添加或不添加干扰素γ阻断抗体的条件。刺激完成后，通过荧光激活细胞分选（FACS）分离得到上皮细胞（CD45⁻、CD31⁻、CD326⁺），随后采用单细胞RNA测序（scRNA-seq）进行分析。本研究共设置三种刺激条件：未刺激组、流感肽联合同型对照抗体刺激组、流感肽联合抗干扰素γ抗体刺激组。整体实验设计为：对气道上皮细胞开展单细胞RNA测序，对比三种刺激条件下的细胞表达差异。测序完成后，基于关键转录因子将总上皮细胞划分为不同细胞亚群。