The effect of G-quadruplex on gene expression by treating S2 cell with different concentration of TMPyP4

In this study, G-quadruplex ligand TMPyP4 was utilized to treat S2 cell and DMSO was served as control. The stabilization role of TMPyP4 will promote the formation of G-quadruplex and then affect gene expression. Identifying whether the effect is positive or negative is important for our understanding of the functionality of non-coding element. We treated S2 cell with different concentration of TMPyP4 (25μmol/L, 50μmol/L, 100μmol/L). We designed three experimental groups and one control group. The experimental group 1 was treated with 25μmol/L alone, the experimental group 2 was treated with 50μmol/L alone, and the experimental group 3 was treated with 100μmol/L alone. The drug solvent DMSO-treated group served as a control group, with 3 replicates per treatment in each group.

本研究采用G-四链体（G-quadruplex）配体TMPyP4处理S2细胞，并以二甲基亚砜（DMSO）作为对照。TMPyP4的稳定化作用可促进G-四链体的形成，进而调控基因表达。明确该效应的正负性，对于理解非编码元件（non-coding element）的功能具有重要意义。本研究使用不同浓度的TMPyP4（25μmol/L、50μmol/L、100μmol/L）处理S2细胞，共设置3个实验组与1个对照组：实验组1仅接受25μmol/L TMPyP4单独处理，实验组2仅接受50μmol/L TMPyP4单独处理，实验组3仅接受100μmol/L TMPyP4单独处理；以DMSO溶剂处理组作为对照组，每组处理均设置3次生物学重复。