Ultrasensitive gamma delta T-cell DNA immune repertoire sequencing with unique molecular identifiers

Immune repertoire sequencing using next-generation sequencing suffers from quantitative biases due to non-uniform amplification, and errors when comparing similar sequences due to polymerase-induces errors. This project contains data generated using a novel ultrasensitive DNA sequencing method to profile the immune repertoire using PCR-introduced unique molecular identifiers. We targeted the T-cell receptor delta (TRD) locus in human gamma delta T-cells, a cell population with importance in colon cancer and immunological diseases such as multiple sclerosis. The project contains sequence data of the TRD locus generated from sorted and unsorted gamma delta T-cells. The material was derived from PBMC donated by healthy individuals. The project also contains data from synthetic reference molecules that were used for assay validation.

基于下一代测序的免疫组库测序技术，因扩增不均一性存在定量偏差，同时在比对相似序列时会因聚合酶诱导的错误引入误差。本数据集采用一种新型超灵敏DNA测序方法生成，该方法借助PCR引入的独特分子标识符（unique molecular identifiers）解析免疫组库。本研究针对人类γδ T细胞中的T细胞受体δ（T-cell receptor delta, TRD）基因座展开，该细胞群在结肠癌及多发性硬化等免疫性疾病中具有重要意义。本数据集包含分选后与未分选的γδ T细胞的TRD基因座测序数据。实验材料来源于健康志愿者捐献的外周血单个核细胞（peripheral blood mononuclear cell, PBMC）。本数据集还包含用于实验验证的合成参考分子相关数据。