Gene expression profile of MHCII+ cell subsets from bone marrow culture with FLT3L or GM-CSF

BM culture comprises of various cell types including dendritic cells and macrophage-like cells. To understand the biology of BM-derived DCs, CD11c+MHCII+ cell subsets from BM cultures with FLT3L or GM-CSF were analyzed and are shown to have distinct transcriptional profiles. Overall design: This study includes data from various DC subsets and macrophages purified by flow cytometry sorting from BM culture with GM-CSF or FLT3L of WT C57BL/6 mice. At least two independent replicates were made for each cell type. Global mRNA expression profiles of the MHCII+ cell subsets from BM culture were done by Illumina Hi-seq 2500. Submitter states that raw data for the cDC2 samples has been lost.

骨髓（Bone Marrow, BM）培养物包含多种细胞类型，涵盖树突状细胞（dendritic cells, DC）与巨噬细胞样细胞。为解析骨髓来源树突状细胞的生物学特性，本研究对经FMS样酪氨酸激酶3配体（FMS-like tyrosine kinase 3 ligand, FLT3L）或粒细胞-巨噬细胞集落刺激因子（granulocyte-macrophage colony-stimulating factor, GM-CSF）诱导的骨髓培养物中的CD11c+MHCII+细胞亚群进行了分析，结果显示其具有独特的转录谱。

总体实验设计：本研究的数据来源于野生型（Wild Type, WT）C57BL/6小鼠经GM-CSF或FLT3L诱导的骨髓培养物，通过流式细胞术分选出的各类树突状细胞亚群及巨噬细胞。每种细胞类型均设置至少2次独立生物学重复。本研究采用Illumina Hi-seq 2500平台完成了骨髓培养物中MHCII+细胞亚群的全基因组mRNA表达谱测序。数据提交者说明，cDC2（经典树突状细胞2, conventional DC2）样本的原始测序数据已丢失。