Next-generation sequencing facilitates quantitative analysis of wild-type peritoneal murine mast cells (PMCs) and their comparison to PMC lines

Spontaneously developed murine PMC lines were compared to naive murine PMCs to elucidate genomic changes and correlate those to phenotypes observed with respect to mast cell effector functions, characteristics and activation of siganling pathways. Moreover, control PMCs will be used to analyze gender differences. Overall design: mRNA profiles of untreated peritoneal mast cells (PMCs) of wild-type (WT) mice (cell lines and naive) were generated by deep sequencing, in triplicate

本研究将自发构建的小鼠腹腔肥大细胞（peritoneal mast cells, PMC）系与未致敏的小鼠腹腔肥大细胞进行对比，旨在阐明其基因组变化，并将这些变化与肥大细胞效应功能、细胞特性及信号通路激活相关的表型建立关联。此外，对照腹腔肥大细胞将用于分析性别差异。实验整体设计：对野生型（wild-type, WT）小鼠未处理的腹腔肥大细胞（包含细胞系与原代未致敏细胞）进行深度测序（deep sequencing），以获取其mRNA表达谱（mRNA profiles），每组设置三次生物学重复。