Digital transformation of herbal medicine: Conversion to biological entity data using tonifying herbal medicine-induced transcriptome sequencing_HT29_batchE

This study aimed to identify transcript expression induced by tonifying herbal medicines (THM) and modes of action (MOA) of THM in HT29 cell line. For the production of THM-induced transcripts, tonifying herbal medicines water extract (THW) and tonifying herbal medicines ethanol extract (THE) were used. Overall design: Comparative gene expression profiling analysis of RNA-seq data for THW (500 ug/mL, 100 ug/mL, and 20 ug/mL, respectively), THE (500 ug/mL, 100 ug/mL, and 20 ug/mL, respectively), positive control (wortmannin and LY294002 10uM, respectively), and vehicle control (2% DMSO). However, in extracts with IC20 of less than 500ug/mL, the concentration at IC20 and the concentration diluted to 1/5 and 1/25 of the IC20 concentration were used, respectively. Please note that the RCM_tonifying_HT29.txt contains processed data for all batch samples and the corresponding data column header is indicated in the sample description field.

本研究旨在鉴定补益类中药（tonifying herbal medicines, THM）诱导的转录本表达，以及补益类中药在HT29细胞系中的作用模式（modes of action, MOA）。为获取补益类中药诱导的转录本，本研究采用补益类中药水提取物（tonifying herbal medicines water extract, THW）与补益类中药乙醇提取物（tonifying herbal medicines ethanol extract, THE）开展实验。实验整体设计：对不同处理组的RNA测序（RNA-seq）数据进行比较基因表达谱分析，处理组包括THW（浓度分别为500 μg/mL、100 μg/mL与20 μg/mL）、THE（浓度分别为500 μg/mL、100 μg/mL与20 μg/mL）、阳性对照组（分别为渥曼青霉素与LY294002，浓度均为10 μM）以及溶剂对照组（2% 二甲基亚砜，DMSO）。但对于半数抑制浓度（IC20）低于500 μg/mL的提取物，实验分别采用其IC20浓度，以及将该浓度稀释至1/5与1/25后的浓度进行。请注意，RCM_tonifying_HT29.txt包含所有批次样本的处理后数据，对应的列标题已在样本说明字段中注明。