Next Generation Sequencing Analysis of Maize Wild Type (WT) and thk1 Mutants Transcriptomes. Next Generation Sequencing Analysis of Maize Wild Type (WT) and thk1 Mutants Transcriptomes

The thk1 mutant, causing multiple aleurone layers, disrupts the NOT1 scaffolding subunit of the CCR4-NOT regulatory complex and dysregulates genes involved in cell division, signaling, differentiation and metabolism. Overall design: Transcriptomes of 19 DAP endosperm from WT (as reference) and mutants of two thk1 alleles, thk1-iso15 and thk1-iso17 were profiled and analyzed. All were in a B73 inbred genetic background and 3 biological replicates were conducted for each.

可诱导产生多糊粉层（aleurone layer）表型的thk1突变体（thk1 mutant），可破坏CCR4-NOT调控复合体（CCR4-NOT regulatory complex）的NOT1支架亚基（NOT1 scaffolding subunit），并异常调控参与细胞分裂、信号转导、细胞分化及新陈代谢的基因。实验设计概述：本研究对遗传背景为B73自交系的野生型（Wild Type，WT，作为对照）以及两个thk1等位基因突变体（thk1-iso15和thk1-iso17）的授粉后19天（days after pollination, DAP）的胚乳转录组进行了测序分析，每组均设置3次生物学重复（biological replicate）。