Table_4_Overexpression of Phosphate Transporter Gene CmPht1;2 Facilitated Pi Uptake and Alternated the Metabolic Profiles of Chrysanthemum Under Phosphate Deficiency.XLSX

Low availability of phosphorus (P) in the soil is the principal limiting factor for the growth of cut chrysanthemum. Plant phosphate transporters (PTs) facilitate acquisition of inorganic phosphate (Pi) and its homeostasis within the plant. In the present study, CmPht1;2 of the Pht1 family was cloned from chrysanthemum. CmPht1;2 is composed of 12 transmembrane domains and localized to the plasma membrane. Expression of CmPht1;2 in roots was induced by Pi starvation. Chrysanthemum plants with overexpression of CmPht1;2 (Oe) showed higher Pi uptake, as compared to the wild type (WT), both under Pi-starvation and Pi-sufficient conditions, and also showed a higher root biomass compared to WT in the Pi-starvation conditions. Seven days after the P-deficiency treatment, 85 distinct analytes were identified in the roots and 27 in the shoots between the Oe1 plant and WT, in which sophorose, sorbitol (sugars), hydroxybutyric acid (organic acids), and ornithine (amino acid) of CmPht1;2 overexpressing chrysanthemum are specific responses to P-starvation.

土壤中有效磷（phosphorus, P）匮乏是限制切花菊生长的首要限制性因子。植物磷酸盐转运蛋白（phosphate transporters, PTs）能够介导植物对无机磷酸盐（inorganic phosphate, Pi）的吸收，并维持其体内的磷稳态。本研究从切花菊中克隆得到Pht1家族成员CmPht1;2。CmPht1;2包含12个跨膜结构域（transmembrane domains），定位于质膜（plasma membrane）。该基因在根系中的表达可被磷饥饿胁迫（Pi starvation）诱导。与野生型（wild type, WT）相比，过表达CmPht1;2的植株（overexpression, Oe）在磷饥饿和磷充足培养条件下均表现出更高的无机磷酸盐吸收效率；且在磷饥饿条件下，其根系生物量（root biomass）也显著高于野生型。缺磷处理7天后，过表达株系Oe1与野生型相比，根系中鉴定出85种差异分析物，地上部中鉴定出27种差异分析物，其中槐糖（sophorose）、山梨醇（sorbitol，糖类）、羟基丁酸（hydroxybutyric acid，有机酸）以及鸟氨酸（ornithine，氨基酸）为CmPht1;2过表达切花菊对磷饥饿胁迫的特异性响应物质。