Identification of the Bacteroides fragilis EcfO regulon 2. Bacteroides fragilis 638R

Genome expression study of Bacteroides fragilis strain 638R comparing a EcfO null mutant with an empty vector to a anti-EcfO null mutant containing the EcfO gene on a plasmid under the control of an IPTG inducible promoter. Overall design: Two chip study with 8 technical replicates on each chip comparing the EcfO overexpressing strain in an anti-EcfO mutant to an empty vector control in a EcfO null mutant.

脆弱拟杆菌（Bacteroides fragilis）菌株638R的基因组表达研究，对比了两类实验菌株：一类为携带空载体的EcfO缺失突变株，另一类为在IPTG诱导型启动子调控下、通过质粒携带EcfO基因的抗EcfO缺失突变株。整体实验设计为双芯片研究，每张芯片设置8次技术重复，用于对比抗EcfO突变株中的EcfO过表达菌株与EcfO缺失突变株中的空载体对照菌株。