PDGFRα+ cells in ESC cultures represent the in vitro equivalent of the pre-implantation primitive endoderm precursors. Mus musculus

Mouse Embryonic Stem Cells (ESCs) express PDGFRα heterogeneously, fluctuating between a PDGFRα+ (PrE-primed) and a Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third subpopulation, expressing epiblast and PrE determinants. Overall design: Three different subpopulatiosn were sorted based on PECAM1/PDGFRa expression and analyzed by NGS

小鼠胚胎干细胞（ESCs）对血小板衍生生长因子受体α（PDGFRα）的表达呈异质性，在PDGFRα阳性（原始内胚层预编程，PrE-primed）与血小板内皮细胞黏附分子1（PECAM1）阳性（上胚层预编程）两种状态间动态波动。两种表面标志物可在同时表达上胚层与原始内胚层决定因子的第三类细胞亚群中共检测到。实验设计概述：基于PECAM1与PDGFRα的表达水平分选得到三种不同的细胞亚群，并通过下一代测序（NGS）完成分析。