In human cells, Staufen1 is double-stranded RNA-binding protein involved in several cellular functions including mRNA localization, translation and decay. We used a genome wide approach to identify an

NIAID Data Ecosystem70

Saccharomyces cerevisiae Raw sequence reads. Saccharomyces cerevisiae

RNA结合蛋白靶标

酵母基因编辑实验

we established an efficient HyperTRIBE (Targets of RNA-binding proteins Identified By Editing) in yeast, by fusing a RBP to the hyper active catalytic domain of human RNA editing enzyme ADAR2 and expr

NIAID Data Ecosystem40

Identification of Rbm25-binding RNAs in bone-marrow-derived macrophages by RIP-seq

RNA结合蛋白靶标

巨噬细胞可变剪接

To identify the potential mRNAs that bound by Rbm25, which might be the direct target of Rbm25 for alternative splicing in murine macrophages. RIP-seq assays were performed using antibody against Rbm2

NIAID Data Ecosystem30

eiCLIP analysis of RBFOX2 in primary mouse hepatocytes

RNA结合蛋白靶标

胆固醇代谢调控

Rbfox2 is required for maintaining hepatic cholesterol levels under high fructose. Here we carry out an enhanced iCLIP (eiCLIP) protocol in order to determine the targets of Rbfox2 in primary mouse h

NIAID Data Ecosystem60

Flag-RBM47 HITS-CLIP in brain metastatic derivatives of MDA-MB-231

RNA结合蛋白靶标

癌症转移机制

Brain metastatic breast cancer cells were subjected to HITS-CLIP to identify the targets of the RNA binding protein RBM47 MDA231-BrM2a is characterized in PMID: 19421193; Related data published toget

NIAID Data Ecosystem40