WUSHEL Involves in the Temperature-dependent Super-double-like Flower Malformation in Carnation (Dianthus caryophyllus L.)

Malformed flowers were found in potted plants of carnation (Dianthus caryophyllus L.) cv. Cherie. There are several malformed phenotypes. In malformed flower (mlf) lines. We hypothesized that the malformation is due to mutation in relevant to morphological and thermal responses. In this study, RNA-seq of young flower buds and whole genome re-sequence of leaves were performed in plants of normal (WT) and mlf lines to verify this hypothesis and seek candidate genes.

在盆栽香石竹（Dianthus caryophyllus L.）栽培品种‘Cherie’中发现畸形花现象。该畸形花存在多种畸形表型，对应的株系被命名为畸形花（malformed flower, mlf）株系。本研究推测，该畸形表型由与形态建成及温度响应相关的基因突变引发。为验证上述假说并筛选候选基因，本研究对野生型（Wild Type, WT）与mlf株系的幼小花芽进行了转录组测序（RNA-seq），并对其叶片开展了全基因组重测序。