Recombinant Eda protein induces supernumerary mammary placodes and upregulates NF-κB reporter expression ex vivo.

(A-D) Stereomicroscope images of E12.5 flank skins of WTC57BL/6 (n = 11) (A), K14-Eda (n = 15) (B), WTNMRI (n = 21) (C) and WTNMRI supplemented with 250 ng/mL of Fc-Eda (n = 42) (D) cultured for two days ex vivo. A and C are control littermates of B and D, respectively. Ectopic buds were observed in 0/32 control, 15/15 K14-Eda, and 38/42 Fc-Eda treated explants. (E-F) NF-κB reporter expression was analysed in E12.5+1d and E12.5+2d explants cultured in the control medium (E) and medium supplemented with 250ng/mL of Fc-Eda (F), by X-gal staining. (Scale bar: 100 μm.)

(A-D) 为体外培养2天的各组小鼠胚胎12.5天（E12.5）侧腹皮肤的体视显微镜图像：其中(A)为WTC57BL/6品系小鼠（样本量n=11），(B)为K14-Eda转基因小鼠（样本量n=15），(C)为WTNMRI品系小鼠（样本量n=21），(D)为添加250 ng/mL Fc-Eda的WTNMRI小鼠外植体（样本量n=42）。(A)与(C)分别为(B)与(D)的对照同窝仔鼠。异位芽在0/32例对照组外植体中未被检出，在15/15例K14-Eda组外植体中均被检出，在38/42例Fc-Eda处理组外植体中被检出。(E-F) 通过X-gal染色技术，分析了对照组培养基(E)与添加250ng/mL Fc-Eda的培养基(F)中培养的E12.5+1d及E12.5+2d外植体的NF-κB报告基因表达情况。（标尺：100 μm）