Norway spruce cold stress roots data set

So far most cold acclimation gene expression studies have addressed short-term responses in herbaceous plant leaves but few have addressed the acclimation responses in roots and even fewer have investigated perennial evergreens such as conifers. RNA-Sequencing data from roots of Norway spruce (Picea abies [L.] Karst.) subjected to cold treatments through a chilling progression (5 degrees Celsius) until 10 days at freeze temperature (-5 degrees Celsius) were analysed and a regulatory network was constructed. Two-year-old Norway spruce seedlings of the seed provenance Lilla Istad-Sweden were planted in pots and grown at 18 degrees Celsius in a 16 h : 8 h, light : dark photoperiod. Forty seedlings were shifted to continuous cold temperature at 5 degrees Celsius for up to ten days. Roots were collected 0, 6, 24 hours and 3 and 10 days after start of the cold treatment. Subsequently the seedlings were exposed to sub-zero freezing temperature at -5 degrees Celsius for another ten days. Samples were taken 6, 24 hours and 3 and 10 days after adaptation of the air temperature to sub-zero. For each time point three replicates were subsequently analysed.

迄今为止，多数植物冷驯化基因表达研究多聚焦于草本植物叶片的短期响应，针对根系冷驯化响应的研究相对匮乏，而以针叶树这类多年生常绿植物为对象的相关研究更是极少。本研究针对挪威云杉（Picea abies [L.] Karst.）的根系开展梯度冷处理：先以5℃低温驯化，再降至-5℃冷冻并持续10天，对其获取的RNA测序（RNA-Sequencing）数据进行分析，并构建了基因调控网络。本研究所用材料为种源来自瑞典利斯塔德（Lilla Istad-Sweden）的2年生挪威云杉实生苗，将其定植于盆钵中，于18℃、16小时光照∶8小时黑暗的光周期条件下培养。随后将40株幼苗转移至5℃持续低温环境中处理至多10天，并于处理开始后的0、6、24小时以及第3天和第10天采集根系样本。后续将幼苗转移至-5℃亚零冷冻环境中继续处理10天，并于气温降至亚零温度后的6、24小时以及第3天和第10天采集样本。每个时间点均设置3次生物学重复用于后续分析。