Transcriptome analysis reveals key gene expression changes in blue catfish sperm in response to cryopreservation. Transcriptome analysis reveals key gene expression changes in blue catfish sperm in response to cryopreservation

Cryopreservation of blue catfish sperm can cause variable embryo hatch rates, which is the limiting factor for hybrid catfish breeding. In this study, we investigated gene expression changes caused by cryopreservation using transcriptome profiles of fresh sperm samples and cryopreserved sperm from the same set of blue catfish. Overall design: Profile of transcriptome-wide gene expression changes in cryopreserved sperm samples (n=3) compared to fresh sperm samples (n=3).

蓝鲶鱼（blue catfish）精子的冷冻保存会导致胚胎孵化率存在差异，这是杂交鲶鱼育种的限制性因素。本研究中，我们利用同一批蓝鲶鱼的新鲜精子样本与冷冻保存精子样本的转录组（transcriptome）谱，探究了冷冻保存所引发的基因表达变化。实验整体设计：对比冷冻保存精子样本（n=3）与新鲜精子样本（n=3）的全转录组基因表达变化谱。