Data_Sheet_2_Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism.CSV

Lactic acid bacteria (LAB) can produce a vast spectrum of antifungal metabolites to inhibit fungal growth. The purpose of this study was to elucidate the antifungal effect of isolated Weissella cibaria BYL4.2 on Penicillium chrysogenum, the antifungal activity of W. cibaria BYL4.2 against P. chrysogenum was evaluated by the superposition method, results showed that it had obviously antifungal activity against P. chrysogenum. Studying the probiotic properties of BYL4.2 and determining it as beneficial bacteria. Furtherly, different treatments were carried out to characterize the antifungal activity of cell-free supernatant (CFS) produced by W. cibaria BYL4.2, and it was shown that the CFS was pH-dependent, partly heat-sensitive, and was not influenced by proteinaceous treatment. The CFS of W. cibaria BYL4.2 was analyzed by high-performance liquid chromatography (HPLC) and found the highest content of lactic acid. Screening of metabolic markers by a non-targeted metabolomics approach based liquid chromatography-mass spectrometry (LC-MS). The results speculated that organic acid especially detected D-tartaric acid was the main antifungal substance of CFS, which could cause the down-regulation of metabolites in the ABC transporters pathway, thereby inhibiting the growth of P. chrysogenum. Therefore, this study may provide important information for the inhibitory mechanism of W. cibaria BYL4.2 on P. chrysogenum, and provide a basis for further research on the antifungal effect of Weissella.

乳酸菌（Lactic acid bacteria, LAB）可产生丰富多样的抗真菌代谢物，用以抑制真菌生长。本研究旨在阐明分离获得的食窦魏斯氏菌（Weissella cibaria）BYL4.2对产黄青霉（Penicillium chrysogenum）的抗真菌效应；采用叠加法评价食窦魏斯氏菌BYL4.2对产黄青霉的抗真菌活性，结果显示其对产黄青霉具有显著抗真菌活性。对菌株BYL4.2的益生菌特性展开研究，并将其鉴定为有益菌株。进一步通过多种处理方式表征食窦魏斯氏菌BYL4.2所产无细胞上清液（cell-free supernatant, CFS）的抗真菌活性，结果表明该CFS具有pH依赖性、部分热敏感性，且不受蛋白类处理的影响。采用高效液相色谱法（high-performance liquid chromatography, HPLC）对食窦魏斯氏菌BYL4.2的无细胞上清液进行分析，发现其中乳酸含量最高。通过基于液相色谱-质谱联用技术（liquid chromatography-mass spectrometry, LC-MS）的非靶向代谢组学方法筛选代谢标志物。研究结果推测，有机酸尤其是检测到的D-酒石酸是该无细胞上清液的主要抗真菌物质，其可导致ABC转运蛋白通路中的代谢产物下调，从而抑制产黄青霉的生长。因此，本研究可为阐明食窦魏斯氏菌BYL4.2对产黄青霉的抑制机制提供重要参考依据，并为后续开展食窦魏斯氏菌抗真菌作用的相关研究奠定基础。