Table 1_New therapeutic targets for endometriosis predicted through mendelian randomization analysis and case-control trials.xlsx

IntroductionEndometriosis is a common chronic gynecological condition that affects approximately 10% of women of reproductive age worldwide.
MethodsThis study utilized large-scale genome-wide association study data and explored the causal relationship between blood metabolites, plasma proteins, and endometriosis via Mendelian randomization and colocalization analysis methods. Clinical pathological data were collected, and hypotheses were validated through experiments such as ELISA, RT-qPCR, and Western blotting.
ResultsRSPO3 and FLT1 were found to be potentially associated with endometriosis within the proteome. External validation and colocalization analysis confirmed the robustness of the association with RSPO3. Blood and tissue samples were collected from clinical patients to assess the accuracy of these predictions.
DiscussionThese results suggest that RSPO3 may be a new target for the treatment of endometriosis, providing a direction for future drug development.

引言
子宫内膜异位症（Endometriosis）是一种常见的慢性妇科疾病，全球范围内约有10%的育龄女性受其影响。
方法
本研究利用大规模全基因组关联研究（genome-wide association study, GWAS）数据，通过孟德尔随机化（Mendelian randomization）与共定位分析（colocalization analysis）方法，探究了血液代谢物、血浆蛋白与子宫内膜异位症之间的因果关联。研究收集了临床病理数据，并通过酶联免疫吸附试验（ELISA）、实时定量聚合酶链式反应（RT-qPCR）以及蛋白质印迹法（Western blotting）等实验对假说进行验证。
结果
本研究在蛋白质组中发现RSPO3与FLT1可能与子宫内膜异位症存在潜在关联。外部验证与共定位分析证实了RSPO3与该病的关联具有稳健性。研究收集了临床患者的血液与组织样本，以评估上述预测结果的准确性。
讨论
上述研究结果表明，RSPO3或可成为子宫内膜异位症治疗的全新靶点，为未来的药物研发提供了方向。