RNA Sequencing of Meloidogyne incognita exposed to Nematicides

In this study, Meloidogyne incognita second stage juveniles were exposed to 4 different nematicides (oxamyl, fluopyram, fluazaindolizine, fluensulfone) in 4 replicates along with a water control for 24-hrs. After 24-hrs, nematodes were flash frozen in liquid nitrogen, total RNA was extracted, and Illumina 100bp single-end read libraries were made, and sequenced on Illumina HiSeq 3000 platform.

本研究中，将南方根结线虫（Meloidogyne incognita）二龄幼虫暴露于4种不同的杀线虫剂（杀线威（oxamyl）、氟吡菌酰胺（fluopyram）、氟吲唑磺菌胺（fluazaindolizine）、氟噻砜（fluensulfone）），设置4次生物学重复并辅以无菌水对照，处理时长为24小时。处理24小时后，将线虫置于液氮中快速冷冻，随后提取总RNA，构建Illumina 100bp单端测序文库，并在Illumina HiSeq 3000测序平台上完成测序。